Bojana M. Kokić1, Ljubica P. Dokić2, Radmilo R. Čolović1, Vojislav V. Banjac1, Rade D. Jovanović3,
Sanja J. Popović1, Jasmina M. Lazarević1 "/> Bojana M. Kokić1, Ljubica P. Dokić2, Radmilo R. Čolović1, Vojislav V. Banjac1, Rade D. Jovanović3,
Sanja J. Popović1, Jasmina M. Lazarević1 "/>

Food & Feed Research

THE POSSIBILITY OF IN VITRO MULTI-ENZYMATIC METHOD APPLICATION FOR THE ASSESSMENT OF THE INFLUENCE OF THERMAL TREATMENTS ON ORGANIC MATTER DIGESTIBILITY OF FEED FOR RUMINANTS

DOI: UDK:
636.085.2:543.38:633.15
JOURNAL No:
Volume 45, Issue 1
PAGES
53-58
KEYWORDS
organic matter digestibility, ruminant, corn, thermal treatment
TOOLS Creative Commons License
Bojana M. Kokić1, Ljubica P. Dokić2, Radmilo R. Čolović1, Vojislav V. Banjac1, Rade D. Jovanović3,
Sanja J. Popović1, Jasmina M. Lazarević1
1University of Novi Sad, Institute of Food Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
2University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
3Institute for Science Application in Agriculture, Bulevar despota Stefana 68b, 11000 Belgrade, Serbia

ABSTRACT

In vitro multi-enzymatic method is a two-step procedure that uses exogenous enzymes for incubation of feed that aim to imitate digestive processes in the animal. It is used for determination of organic matter digestibility (OMD) of various feedstuffs and complete mixtures used in ruminant nutrition. The aim of the present work was to determine whether this in vitro multi-enzymatic method can be applied for the assessment of the influence of thermal treatments on OMD of feed for ruminants. Samples of corn and complete mixture for cows (based on corn) were subjected to pelleting, steam flaking, micronization and extrusion. Statistical analysis of the results did not show significant increase of corn and complete mixture OMD after various thermal treatments compared to untreated samples, which can be explained by high digestibility of corn itself (exceeds 90%). Based on obtained results it can be concluded that applied in vitro method is not suitable for the assessment of the influence of thermal treatments on OMD of corn and corn based mixture, while future research should be broadened to analysis of samples with lower OMD, such as sorghum, oats and barley.



Download full article PDF
DOWNLOAD PDF




REFERENCES

  1. Adesogan, A.T. (2002). What are feeds worth?: A critical evaluation of selected nutritive value methods. 13th Annual Florida Ruminant Nutrition Symposium, Proceedings, pp. 33-47.
  2. Akerlind, M., Weisbjerg, M., Eriksson, T., To-gersen, R., Uden, P., Olafsson, B., Harstad, O., Volden, H. (2011). Feed analysis and digestion methods. In Norfor – the Nordic feed evaluation system. Ed. H. Volden, Wageningen Academic Publishers, Wageningen, The Netherlands, pp. 41-54.
  3. Aufrere, J., Michalet-Doreau, B. (1988). Comparison of methods for predicting digestibility of feeds. Animal Feed Science and Technology, 20, 203-218.
  4. Bailoni, L., Mantovani, R., Pagnin, G., Schia-von, S. (2006). Effects of physical treatments on the resistant starch content and in vitro organic matter digestibility of different cereals in horses. Livestock Science, 100, 14-17.
  5. Cooper, R., Milton, C., Klopfenstein, T.J., Scott, T., Wilson, C., Mass, R. (2002). Effect of corn processing on starch digestion and bacterial crude protein flow in finishing cattle. Journal of Animal Science, 80, 797-804.
  6. Getachew, G., Blümmel, M., Makkar, H.P.S., Becker, K. (1998). In vitro gas measuring techniques for assessment of nutritional quality of feeds: a review. Animal Feed Science and Technology, 72, 261-281.
  7. Gosselink, J.M.J., Dulphy, J.P., Poncet, C., Jailler, M., Tamminga, S., Cone, J.W. (2004). Prediction of forage digestibility in ruminants using in situ and in vitro techniques. Animal Feed Science and Technology, 115, 227-246.
  8. Hvelplund, T., Riis Weisbjeng, M., Soegaard, K. (1999). Use of in vitro digestibility methods to estimate in vivo digestibility of straws. 26th Scientific Conference of the Tanzania Society of Animal Production, Proceedings, pp. 70-79.
  9. Jones, D.I.H., Theodorou, M.K. (2000). Enzyme techniques for estimating digestibility. In Forage evaluation in ruminant nutrition. Eds. D.I. Givens, E. Owen, R.F.E. Axford, H.M. Omed, CABI Publishing, Oxon, UK, pp. 155-173.
  10. Kokić, B., Palić, D., Ivanov, D., Lević, J., Spa-sevski, N., Đuragić, O., Čabarkapa, I. (2013). Modification of in vitro multi-enzymatic method for determining the organic matter digestibility of feeds. Agro FOOD Industry Hi-Tech, 24, 59-61.
  11. Lopez, S. (2005). In vitro and in situ techniques for estimating digestibility. In Quantitative Aspects of Ruminant Digestion and Metabolism. Eds. J. Dijkstra, J.M. Forbes, J. France, 2nd edn. CAB International, Wallingford, UK, pp. 87-121.
  12. Menke, K.H., Steingass, H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development, 28, 7-55.
  13. Palić, D., Leeuw, K.-J. (2009). Comparison of three in vitro methods for determining and predicting the organic matter digestibility of complete diets for ruminants. Acta periodica technologica, 40, 79-86.
  14. Palić, D., Muller, H. (2006). Prediction of the in vivo organic matter digestibility of feedstuffs for ruminants using in vitro techniques. Savremena poljoprivreda, 55, 127-132.
  15. Pojić, M., Palić, D., Mastilović, J., Janić-Hajnal, E. (2008). The introduction of a method for determination of organic matter digestibility in feeds into routine laboratory practice. Food Processing, Quality and Safety, 35, 151-155.
  16. Qiao, F.-Q., Wang, F., Ren, L.-P., Zhou, Z.-M., Meng, Q.-X., Bao, Y.-H. (2015). Effect of steam-flaking on chemical compositions, starch gelatinization, in vitro fermentability, and energetic values of maize, wheat and rice. Journal of Integrative Agriculture, 14, 949-955.
  17. Tilley, J.M.A., Terry, R.A. (1963). A two-stage technique for the in vitro digestion of forage crops. Grass and Forage Science, 18, 104-111.
  18. Weisberg, R., Hvelplund, T. (1993). Estimation of net energy content in feeds for cattle, Report No. 3/1993, National Institute of Animal Science, Tjele, Denmark.






INSTITUTE
OF FOOD
TECHNOLOGY
IN NOVI SAD
www.fins.uns.ac.rs