Food & Feed Research


Volume 45, Issue 1
Clostridium perfringens, toxin genotyping, animal feed, multiplex PCR assay, cpa gen, cpb2 gen
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Dubravka S. Milanov1*, Tamaš R. Petrović1, Dalibor S. Todorović1,
Nevenka R. Aleksić2, Ivana S. Čabarkapa3
1 Scientific Veterinary Institute „Novi Sad“, 21000 Novi Sad, Rumenački put 20, Serbia
2 University of Belgrade, Faculty of Veterinary Medicine, 11000 Belgrade, Bulevar oslobođenja 18,
3 University of Novi Sad, Institute of Food Technology, 21000 Novi Sad, Bulevar cara Lazara 1, Serbia


Clostridium perfringens is a Gram-positive, endospore-forming, anaerobic rod, ubiquitous in nature. C. perfringens strains can produce about 17 toxins. Many of them can lead to miscellaneous diseases, among which the enteric ailment may be the most common and is of utmost importance. In the present work 34 strains of C. perfringens isolated from feed and one from a cow suspected to have died of clostridial infection were subjected to molecular analysis. In order to detect the genotypes, the following genes coding for toxins were targetted: cpa, cpb, cpb2, cpe, etx and iap. The multiplex PCR assay revealed that all C. perfringens isolates from animal feed were of type A and b2-toxinogenic type A strains, possessing only the cpa (n=21), or both the cpa and the cpb2 genes (n=13). The importance of C. perfringens toxins a and b-2 in the pathogenesis of enterotoxemia is discussed and the regulation on the detection of this bacteria in animal feed questioned. The use of PCR in practise could enable the toxin-genotyping of C. perfringens isolates and, thus, provide a real basis for the establishment of maximum acceptable limits of this bacteria in feed.

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