Food & Feed Research


Volume 46, Issue 1
biopolymer film, pumpkin oil cake, composite, synthesis, characterisation
TOOLS Creative Commons License
Nevena M. Hromiš*, Senka Z. Popović, Danijela Z. Šuput, Sandra N. Bulut, Vera L. Lazić
University of Novi Sad, Faculty of Technology, 21000 Novi Sad, Bulevar cara Lazara 1, Serbia


Packaging is inseparable retainer of almost all food products. However, most of produced packaging ends as packaging waste after consumption of the product. Increasing amounts of packaging waste that should be managed represents serious challange of every modern society. There are many different approaches to addres this subject, amongst which biodegradable, natural biopolymer-based or even edible packaging holds considerable potential. In this paper, a by-product of edible oil industry, left after completed extraction by cold pressing of oil from hulles of pumpkin seeds, was used to produce biopolymer packaging films. Pumpkin oil cake was used to produce composite bio-based films. Different filtration of film forming suspension was applied in order to test composite film production using different filtration fractions, leading to higher process yield and minimizing waste. In addition, films were casted on surface, about ten times larger comparing to the cast surface typically reported for these types of films in the literature, in order to test the possibility for commercial production and scale up process. Also, casted mass of film forming suspension was varied in order to define minimal casting mass per unit area. Presented results showed that biopolymer films based on the pumpkin oil cake can be successfully produced in sheets (50x35 cm), compared to films earlier produced in the form of discs with diameter 12 cm. Different filtration fractions from initial film forming suspension can be used for film formation, leading to increased production yield. Different filtration fractions lead to different film properties that should be adjusted according to selected application. Casted mass of film forming suspension was successfully decreased (comparing to earlier literature data) without compromising film functional properties and minimal casting mass was defined as 26 g/m2.

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  1. ASTM D882-10 (2010). Standard Test Method for Tensile Properties of Thin Plastic Sheeting. American Society for Testing and Materials International, West Conshohocken, Pennsylvania.
  2. Bamdad, F., Goli, A.H., Kadivar, M. (2006). Preparation and characterization of proteinous film from lentil (Lens culinaris): Edible film from lentil (Lens culinaris). Food Research International, 39, 106–111.
  3. Bertelsen, G., Skibsted, L.H. (1987). Photooxidation of oxymyoglobin. wavelength dependence of quantum yields in relation to light discoloration of meat. Meat Science, 19, 243-251.
  4. Bigi, A., Panzavolta, S., Rubini, K. 2004). Relationship between triple-helix content and mechanical properties of gelatin films. Biomaterials, 25, 5675-5680.
  5. Böhner, N., Hösl, F., Rieblinger, K., Danzl, W. (2014). Effect of retail display illumination and headspace oxygen concentration on cured boiled sausages. Food Packaging and Shelf Life 1, 131–139.
  6. Böhner, N., Rieblinger, K. (2016). Impact of different visible light spectra on oxygen absorption and surface discoloration of bologna sausage. Meat Science, 121, 207–209
  7. Bourtoom, T., Chinnan, M.S., Jantawat, P., Sanguandeekul, R. (2006). Effect of plasticizer type and concentration on the properties of edible film from watersoluble fish proteins in surimi wash-water. Food Science and Technology International, 12 (2), 119– 126.
  8. Cao, N., Fu, Y., He, J. (2007). Preparation and physical properties of soy protein isolate and gelatin composite films. Food Hydrocolloids, 21, 1153–1162.
  9. Choi, W.S., Han, J.H. (2001). Physical and mechanical properties of pea-protein-based edible films. Journal of Food Science, 66, 319–322.
  10. Delgado, M., Felix, M., Bengoechea, C. (2018). Development of bioplastic materials: From rapeseed oil industry by products to added-value biodegradable biocomposite materials. Industrial Crops and Products, 125, 401–407.
  11. Directive (EU) 2018/852 (2018). Directive (EU) 2018/852 of the European parliament and of the Council amending Directive 94/62/EC on packaging and packaging waste. Official Journal of the European Union, L 150/141.
  12. Fairley, P, Monahan, F., German, B., Krochta, J. (1996). Mechanical properties and water vapor permeability of edible films from whey protein isolate and sodium dodecyl sulfate. Journal of Agricultural and Food Chemistry, 44, 438-443.
  13. Hromiš, N. (2015). Development of biodegradable active packaging material from chitosan: synthesis, optimisation of properties, characterisation and application. PhD Thesis, Faculty of Technology, University of Novi Sad, Serbia.
  14. Intawiwat, N., Myhre, E., Øysæd, H, Jamtvedt, S., Kvalva, M.P. (2012). Packaging materials with tailor made light transmission properties for food protection. Polymer Engineering and Science, 52 (9), 2015-2024.
  15. ISO 2528:1995 (1995). Sheet materials –Determination of water vapour transmission rate – Gravimetric (dish) method. International Organisation for Standardisation, Geneva, Switzerland.
  16. Jongjareonrak, A., Benjakul, S., Visessanguan, W., Tanaka, M. (2006). Effects of plasticizers on the properties of edible films from skin gelatin of bigeye snapper and brownstripe red snapper. European Food Research Technology, 222, 229–235.
  17. Kim, H.W., Kim, K.M., Ko, E.J., Lee, S.K., Ha, S.D., Song, K.B., Park, S.K., Kwon, K.S., Bae, D.H. (2004). Development of antimicrobial edible film from defatted soybean meal fermented by Bacillus subtilis. Journal of Microbiology and Biotechnology, 14 (6), 1303- 1309.
  18. Kim, H.W., Ko, E.J., Ha, S.D., Song, K.B., Park, S.K., Chung, D.H., Youn, K.S., Bae, D.H. (2005). Physical, mechanical and antimicrobial properties of edible films produced from defatted soybean meal fermented by Bacillus subtilis. Journal of Microbiology and Biotechnology, 15 (4), 815-822.
  19. Lee, H., Min, S. (2013). Antimicrobial edible defatted soybean meal-based films incorporating the lactoperoxidase system. LWT - Food Science and Technology, 54, 42-50.
  20. Lin, C. S.K., Koutinas, A., Stamatelatou, K., Mubofu, E., Matharu, A., Kopsahelis, N., Pfaltzgraff, L., Clark, J., Papanikolaou, S., Kwan, T.H., Luque, R. (2014). Perspective: Current and future trends in food waste valorization. Biofuels, Bioproducts and Biorefining, 8, 686–715.
  21. Liu, L.S., Liu, C.K., Fishman, M., Hicks, K. (2007). Composite Films from Pectin and Fish Skin Gelatin or Soybean Flour Protein. Journal of Agricultural and Food Chemistry, 55 (6), 2349-2355.
  22. Lourdin, D., Coignard, L., Bizot, H. and Colonna, P. (1997) Influence of Equilibrium Relative Humidity and Plasticizer Concentration on the Water Content and Glass Transition of Starch Materials. Polymer, 38, 5401-5406.
  23. Mariniello, L., Di Pierro, P., Esposito, C., Sorrentino, A., Masi, P., Porta, R. (2003). Preparation and mechanical properties of edible pectin/soy flour films obtained in the absence or presence of transglutaminase. Journal of Biotechnology, 102, 191-198.
  24. Martins, J. T., Cerqueira, M.A., Vicente A.A. (2012). Influence of -tocopherol on physicochemical properties of chitosan-based films. Food Hydrocolloids, 27, 220-227.
  25. McHugh, T.H., Krochta, Ј. (1994). Water vapor permeability properties of edible whey protein-lipid emulsion films. Journal of the American Oil Chemists’ Society, 71 (3), 307–312.
  26. Mortensen, G., Sørensen, J., Stapelfeldt, H. (2002). Effect of light and oxygen transmission. Characteristics of packaging materials on photo-oxidative quality changes in semi-hard Havarti cheeses. Packaging Technology and Science, 15, 121-127.
  27. Oh, Y.A., Roh, S.H., Min, S. (2016). Cold plasma treatments for improvement of the applicability of defatted soybean meal-based edible film in food packaging. Food Hydrocolloids, 58, 150-159.
  28. OriginPro 8 SR2 (Scientific graphing and data analysis software) (2001). v.8.0891(B891)., OriginLab Corporation, Northampton, MA, USA (
  29. Pérez-Gago, M., Krochta, Ј. (2001). Lipid particle size effect on water vapor permeability and mechanical properties of whey protein/beeswax emulsion films. Journal of Agricultural and Food Chemistry, 49, 996- 1002.
  30. Popović, S. (2013). The study of production and characterization of biodegradable composite films based on plant proteins. PhD Thesis, Faculty of Technology, University of Novi Sad, Serbia.
  31. Popović, S., Lazić, V., Hromiš, N., Šuput, D., Bulut, S. (2018). Biopolymer packaging materials for food shelf-life prolongation. In Vol. 20: Biopolymers for Food Design, Handbook of Food Bioengineering. Eds. A.M. Grumezescu, A.M. Holban, Academic Press, Elsevier, United Kingdom, pp. 223-277.
  32. Popović, S., Peričin, D., Vaštag, Ž., Popović, Lj., Lazić, V. (2011). Evaluation of edible film-forming ability of pumpkin oil cake; effect of pH and temperature. Food Hydrocolloids, 25 (3) 470-476.
  33. Rhim, J.W., Gennadios, A., Weller, C.L., Cazeirat, C., Hanna, M.A. (1998). Soy protein isolate-dialdehydestarch films. Industrial Crops and Products, 8, 195- 203.
  34. Rubilar, J.F., Cruz, R.M.S., Silva, H.D., Vicente, A.A., Khmelinskii, I., Vieira, M.C. (2013). Physico-mechanical properties of chitosan films with carvacrol and grape seed extract. Journal of Food Engineering, 115, 466–474.
  35. Vieira, M.G.A., da Silva, M.A., dos Santos, L.O., Beppu, M.M. (2011). Natural-based plasticizers and biopolymer films: A review. European Polymer Journal, 47, 254–263.