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Food & Feed Research

PRODUCTION OF BIOBACTERICIDES FOR CRUCIFERS BLACK ROT CONTROL: EFFECT OF NITROGEN SOURCES

DOI: UDK:
632.937+632.953]:635.35
JOURNAL No:
Volume 47, Issue 1
PAGES
13-22
KEYWORDS
Biobactericide production, Bacillus subtilis, Nitrogen source, Black rot, Xanthomonascam-pestris
TOOLS Creative Commons License
Jovana A. Grahovac, Jelena M. Dodić Ida E. Zahović*, Zorana Z. Rončević, Jovana J. Đuran, Ivana Ž. Mitrović,
University of Novi Sad, Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, Bulevar cara Lazara 1, Novi Sad 21000, Serbia


ABSTRACT

The aim of this study was to examine the effects of variation of organic (yeast extract, soybean flour and peptone) and inorganic nitrogen sources (NaNO2, KNO3 and (NH4)2HPO4) in medium for biobactericides production by Bacillus subtilis ATCC 6633. The antibacterial activity of samples against the reference strain Xanthomonas campestris ATCC 13951 and different Xanthomonas strains isolated from cabbage, kale and cauliflower was determined in vitro by disc-diffusion method. Following statistical analysis, it was concluded that both nitrogen sources have statistically significant effect on biobactericides production under applied conditions whereby the influence of inorganic nitrogen is more pronounced. The results of Duncan's test suggested that the highest biosynthesis of antibacterial compounds was achieved in both media, with or without yeast extract, when NaNO2 was used as an inorganic nitrogen source. The results from this study can be used in further research to improve biobactericides production by the reference Bacillus subtilis strain.



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