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Volume 45, Issue2
pumpkin oil cake, millet bread, dough rheological properties, sensory quality
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Jelena M. Tomić*1, Aleksandra M. Torbica1, Miona M. Belović1, Ljiljana M. Popović2, Jelena C. Čakarević2, Danica M. Savanović3, Aleksandra R. Novaković1, Karolina A. Mocko Blažek1
1 University of Novi Sad, Institute of Food Technology, 21000 Novi Sad, Bulevar cara Lazara 1, Serbia
2 University of Novi Sad, Faculty of Technology, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia
3 University of Banja Luka, Faculty of Technology, 78000 Banja Luka, Bulevar vojvode Stepe Stepanovića 73, Bosnia and Herzegovina


The objective of this study was to evaluate the potential of pumpkin oil cake protein isolate in production of millet bread. For that purpose, breads were created by substitution of millet flour with proteins at 5, 10 and 15% level. Dough rheological properties and both physical and sensory characteristics of obtained bread were determined. The increase in pumpkin oilseed cake protein (POCP) concentration influenced increase in dough viscosity, as determined using farinograph and fundamental rheological measurements. This is additionally confirmed by lower elasticity of supplemented breads as determined by texture analysis and sensory panel. Substitution of millet flour with POCP at all tested levels did not exhibit any influence on bread specific volume. However, 24 h after baking, breads supplemented with higher amount of POCP showed less pronounced hardening of the crumb, indicating that these proteins might retard starch retrogradation. The supplementation of millet bread with POCP had several beneficial effects on the sensory quality of bread, such as loss of bitter taste and aftertaste originating from millet flour. Additionally, bread granularity decreased and bread dissolving speed in mouth increased along with the increase in POCP concentration.

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  1. AACC (2017). AACC Approved Methods of Analysis, 11th Ed., AACC International, St. Paul, MN, USA, Method 80-68.01 Determination of Reducing Sugars–Schoorl; Retrieved July 2, 2018 from https://aaccipublications.aaccnet.org.
  2. Aiking, H. (2011). Future protein supply. Trends in Food Science and Technology, 22 (2-3), 112- 120.
  3. Annor, G.A., Tyl, C., Marcone, M., Ragaee, S., Marti, A. (2017). Why do millets have slower starch and protein digestibility than other cereals? Trends in Food Science and Technology, 66, 73-83.
  4. AOAC (2000). Official Methods of Analysis, 17th Ed. The Association of Official Analytical Chemists, Gaithersburg, MD, USA, Methods 925.10, 950.36, 935.38.
  5. Callejo, M.J. (2011). Present situation on the descriptive sensory analysis of bread. Journal of Sensory Studies, 26 (4), 255-268.
  6. Demirkesen, I., Mert, B., Sumnu, G., Sahin, S. (2010). Rheological properties of gluten-free bread formulations. Journal of Food Engineering, 96 (2), 295-303.
  7. Duodu, K.G., Nunes, A., Delgadillo, I., Parker, M.L., Mills, E.N.C., Belton, P.S., Taylor, J.R.N. (2002). Effect of grain structure and cooking on sorghum and maize in vitro protein digestibility. Journal of Cereal Science, 35 (2), 161-174.
  8. Fruhwirth, G.O., Hermetter, A. (2007). Seeds and oil of the Styrian oil pumpkin: Components and biological activities. European Journal of Lipid Science and Technology, 109 (11), 1128- 1140.
  9. Gallagher, E., Gormley, T.R., Arendt, E.K. (2003). Crust and crumb characteristics of gluten free breads. Journal of Food Engineering, 56 (2-3), 153-161.
  10. Gallagher, E., Gormley, T.R., Arendt, E.K. (2004). Recent advances in the formulation of gluten-free cereal-based products. Trends in Food Science and Technology, 15 (3-4), 143- 152.
  11. Giuberti, G., Rocchetti, G., Sigolo, S., Fortunati, P., Lucini, L., Gallo, A. (2018). Exploitation of alfalfa seed (Medicago sativa L.) flour into gluten-free rice cookies: Nutritional, antioxidant and quality characteristics. Food chemistry, 239, 679-687.
  12. ISO (1994). Sensory analysis - Identification and selection of descriptors for establishing a sensory profile by a multidimensional approach. ISO 11035, International Organization for Standardization, Geneva, Switzerland.
  13. ISO (1997). Determination of starch content, ISO 10520 International Organization for Standardization, Geneva, Switzerland, Ewers polarimetric method.
  14. ISO (2003). Sensory analysis - Guidelines for the use of quantitative response scales. ISO 4121, International Organization for Standardization, Geneva, Switzerland.
  15. ISO (2015). Sensory analysis - General guidance for the design of test rooms. ISO 8589 (Amendment 1:2014), International Organization for Standardization, Geneva, Switzerland.
  16. Marco, C., Rosell, C.M. (2008). Breadmaking performance of protein enriched, gluten-free breads. European Food Research and Technology, 227 (4), 1205-1213.
  17. Mir, S.A., Shah, M.A., Naik, H.R., Zargar, I.A. (2016). Influence of hydrocolloids on dough handling and technological properties of glutenfree breads. Trends in Food Science and Technology, 51, 49-57.
  18. Mirabella, N., Castellani, V., Sala, S. (2014). Current options for the valorization of food manufacturing waste: a review. Journal of Cleaner Production, 65, 28-41.
  19. Ogunronbi, O., Jooste, P.J., Abu, J.O., Van der Merwe, B. (2011). Chemical composition, storage stability and effect of cold‐pressed flaxseed oil cake inclusion on bread quality. Journal of Food Processing and Preservation, 35 (1), 64-79.
  20. Pojić, M., Dapčević Hadnađev, T., Hadnađev, M., Rakita, S., Brlek, T. (2015). Bread supplementation with hemp seed cake: A by‐product of hemp oil processing. Journal of Food Quality, 38 (6), 431-440.
  21. Popović, L.M., Peričin, D.M., Vaštag, Ž.G., Popović, S.Z. (2013). Optimization of transglutaminase cross-linking of pumpkin oil cake globulin; improvement of the solubility and gelation properties. Food and Bioprocess Technology, 6 (4), 1105-1111.
  22. Popović, S., Peričin, D., Vaštag, Ž., Popović, L., Lazić, V. (2011). Evaluation of edible film-forming ability of pumpkin oil cake; effect of pH and temperature. Food Hydrocolloids, 25 (3), 470-476.
  23. Rodrigues, I.M., Coelho, J.F., Carvalho, M.G. V. (2012). Isolation and valorisation of vegetable proteins from oilseed plants: Methods, limitations and potential. Journal of Food Engineering, 109 (3), 337-346.
  24. Ronda, F., Villanueva, M., Collar, C. (2014). Influence of acidification on dough viscoelasticity of gluten-free rice starch-based dough matrices enriched with exogenous protein. LWT-Food Science and Technology, 59 (1), 12-20.
  25. Shewry, P.R., Halford, N.G. (2002). Cereal seed storage proteins: structures, properties and role in grain utilization. Journal of Experimental Botany, 53 (370), 947-958.
  26. Tarek-Tilistyák, J., Agócs, J., Lukács, M., Dobró-Tóth, M., Juhász-Román, M., Dinya, Z., Jekő, J. Máthé, E. (2014). Novel breads fortified through oilseed and nut cakes. Acta Alimentaria, 43 (3), 444-451.
  27. Vaštag, Ž., Popović, L., Popović, S., Krimer, V., Peričin, D. (2011). Production of enzymatic hydrolysates with antioxidant and angiotensin-I converting enzyme inhibitory activity from pumpkin oil cake protein isolate. Food Chemistry, 124 (4), 1316-1321.
  28. Witczak, T., Juszczak, L., Ziobro, R., Korus, J. (2017). Rheology of gluten-free dough and physical characteristics of bread with potato protein. Journal of Food Process Engineering, 40 (3), e12491.
  29. Ziobro, R., Witczak, T., Juszczak, L., Korus, J. (2013). Supplementation of gluten-free bread with non-gluten proteins. Effect on dough rheological properties and bread characteristic. Food Hydrocolloids, 32 (2), 213-220.