Show simple item record

dc.contributor.authorTodorić, Olja
dc.contributor.authorPezo, Lato
dc.contributor.authorŠarić, Ljubiša
dc.contributor.authorKolarov, Violeta
dc.contributor.authorVarga, Ana
dc.contributor.authorČabarkapa, Ivana
dc.contributor.authorKocić Tanackov, Sunčica
dc.date.accessioned2023-07-15T21:27:13Z
dc.date.available2023-07-15T21:27:13Z
dc.date.issued2023-06
dc.identifier.citationTodorić, O.; Pezo, L.; Šarić, L.; Kolarov, V.; Varga, A.; Čabarkapa, I.; Kocić-Tanackov, S. Comparison of the Efficiency of Selected Disinfectants against Planktonic and Biofilm Populations of Escherichia coli and Staphylococcus aureus. Microorganisms 2023, 11, 1593. https://doi.org/10.3390/ microorganisms11061593en_US
dc.identifier.issn2076-2607
dc.identifier.urihttp://oa.fins.uns.ac.rs/handle/123456789/281
dc.description.abstracthe aim of this study is to compare the efficacy of selected food disinfectants on planktonic populations of Staphylococcus aureus and Escherichia coli and on the same microorganisms (MOs) incorporated in a biofilm. Two disinfectants were used for treatment: peracetic acid-based disinfectant (P) and benzalkonium chloride-based disinfectant (D). Testing of their efficacy on the selected MO populations was performed using a quantitative suspension test. The standard colony counting procedure was used to determine their efficacy on bacterial suspensions in tryptone soy agar (TSA). The germicidal effect (GE) of the disinfectants was determined based on the decimal reduction ratio. For both MOs, 100% GE was achieved at the lowest concentration (0.1%) and after the shortest exposure time (5 min). Biofilm production was confirmed with a crystal violet test on microtitre plates. Both E. coli and S. aureus showed strong biofilm production at 25 °C with E. coli showing significantly higher adherence capacity. Both disinfectants show a significantly weaker GE on 48 h biofilms compared to the GE observed after application of the same concentrations on planktonic cells of the same MOs. Complete destruction of the viable cells of the biofilms was observed after 5 min of exposure to the highest concentration tested (2%) for both disinfectants and MOs tested. The anti-quorum sensing activity (anti-QS) of disinfectants P and D was determined via a qualitative disc diffusion method applied to the biosensor bacterial strain Chromobacterium violaceum CV026. The results obtained indicate that the disinfectants studied have no anti-QS effect. The inhibition zones around the disc therefore only represent their antimicrobial effect.en_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200222/RS// info:eu-repo/grantAgreement/MESTD/inst-2020/200051/RS// info:eu-repo/grantAgreement/PSHESR/apv-2020/3163/RS//
dc.rightsopenAccess
dc.subjectquaternary ammonium compounden_US
dc.subjectperacetic aciden_US
dc.subjectbiofilmen_US
dc.subjectEscherichia colien_US
dc.subjectStaphylococcus aureusen_US
dc.subjectChromobacterium violaceumen_US
dc.subjectquorum sensingen_US
dc.titleComparison of the Efficiency of Selected Disinfectants against Planktonic and Biofilm Populations of Escherichia coli and Staphylococcus aureusen_US
dc.typeArticleen_US
dc.typeinfo:eu-repo/semantics/article
dc.identifier.wos001014852700001
dc.identifier.doi10.3390/microorganisms11061593


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record